![]() There are also various other background subtraction algorithms and you would have to decide, which one is best for your purpose. to deal with uneven brightness across an image) you could use the ‘Subtract Background’ command in ImageJ (found under Process), which uses a rolling ball algorithm for background subtraction. B- I started this thread to have a way to display a Composite with bright-background LUTs. So probably best to only do this on a copy of the original image.Īlso, if you actually want to properly subtract the background (e.g. Here’s a summary of how it went from what I understood: A- Original ImageJ Composite mode is SUM (with clipping at 255), good for dark-background LUTs, but generates an all-white image when using bright-background LUTs. When you click ‘Apply’ in the brightness/contrast window it will actually change the pixel values and the pixels with the value 20 will now have the value 0, while pixels with the value 130 will now have the value 255.īe careful with that as this can’t be reversed and you loose information contained within the pixel values outside your display range. However, the values of the pixels are unchanged. This will adjust the display of the image so that pixels with the value 20 are displayed as black and pixels with the value 130 as white. Let’s say you set the minimum of your display range to 20 and the maximum to 130 in an 8-bit grey scale image. If you want to make these changes permanent so that they appear when opening the image ith a different programme, you need to click the ‘Apply’ button in the Brightness/Contrast contrast control panel, which will actually change the pixel values of the image so that they are mapped to your new display range. When you re-open the same image in Fiji, it remembers your brightness settings for that image, but this may not be the case if you open the image in another programme. ![]() Adjusting the brightness/contrast does not alter the values of the pixels (you can check this by making a selection and measure its average intensity before and after adjusting the brightness). ![]() Sounds like you are just adjusting the display properties without altering the actual pixel values (‘adjusting brightness to reduce background’).
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